Analysis Note

Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.

Application

Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by: Western blotting using the Anti-HA antibodySilver staining (or similar protein stain) In coimmunoprecipitation.

Use Anti-HA Affinity Matrix for: Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extractsAffinity column purification of HA-tagged proteins from crude protein extracts

Features and Benefits

Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity. Use nondenaturing conditions for elution of your HA-tagged protein of choice. Purify even rarely expressed HA-tagged proteins. Highly specific to YPYDVPDYA. Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion. Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.

General description

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)

Immunogen

Amino acids 98-106 from the human influenza virus hemagglutinin protein

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.

Quality

Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.

Specificity

Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).